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human β ngf  (R&D Systems)


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    R&D Systems human β ngf
    Human β Ngf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+ngf/Recombinant+Human+beta-NGF+Protein/pm41999764-275-45-47
    Average 95 stars, based on 111 article reviews
    human β ngf - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Optimising parameters for the differentiation of SH-SY5Y cells to study cell adhesion and cell migration
    Article Snippet: .. Recombinant NGF was purchased from R&D Systems Europe Ltd. (Abington, UK). .. Recombinant IGF-1 was purchased from Peprotech Inc. (Rocky Hill, NJ).

    Article Title: RACK1 promotes neurite outgrowth by scaffolding AGAP2 to FAK.
    Article Snippet: RACK1 binds proteins in a constitutive or transient manner and supports signal transmission by engaging in diverse and distinct signalling pathways.. The emerging theme is that RACK1 functions as a signalling switch, recruiting proteins to form distinct molecular complexes.. In focal adhesions, RACK1 is required for the regulation of FAK activity and for integrating a wide array of cellular signalling events including the integration of growth factor and adhesion signalling pathways.

    Article Title: NGF from pancreatic stellate cells induces pancreatic cancer proliferation and invasion by PI3K/AKT/GSK signal pathway
    Article Snippet: Antibodies against MMP‐9, vimentin, E‐cadherin, NGF, TrkA, p‐AKT, AKT, GSK and p‐GSK were purchased from Abcam. .. Recombinant NGF was obtained from R&D Systems. .. Total RNA was extracted using TRIzol (Invitrogen), and cDNA was synthesized using a PrimeScript RT Reagent Kit (TaKaRa).

    Article Title: Ku86 is important for TrkA overexpression-induced breast cancer cell invasion.
    Article Snippet: .. Recombinant NGF was from R&D Systems (Lille, France). .. Nucleofection reagents were from Amaxa Biosystems (Lonza, Cologne, Germany).

    Article Title: Regulation of Autocrine Signaling in Subsets of Sympathetic Neurons Has Regional Effects on Tissue Innervation
    Article Snippet: .. Recombinant NGF was obtained from R&D Systems (Minneapolis, USA). .. Function blocking antibodies to CD40 and CD40L were obtained from BD Pharmingen (Oxford, UK, catalogue number 553721) and Abcam (Cambridge, UK, catalogue number ab99895), respectively, and the corresponding isotype control antibodies were obtained from BD Pharmingen (catalogue number 553957) and Abcam (catalogue number ab18426).

    Article Title: Regulation of Autocrine Signaling in Subsets of Sympathetic Neurons Has Regional Effects on Tissue Innervation
    Article Snippet: .. Recombinant NGF was obtained from R&D Systems (Minneapolis, USA). .. Function blocking antibodies to CD40 and CD40L were obtained from BD Pharmingen (Oxford, UK, catalogue number 553721) and Abcam (Cambridge, UK, catalogue number ab99895), respectively, and the corresponding isotype control antibodies were obtained from BD Pharmingen (catalogue number 553957) and Abcam (catalogue number ab18426).

    Article Title: Fibroblasts neurotrophin signaling sustains pathological vascular maturation in rheumatoid arthritis
    Article Snippet: .. For neurotrophin (NT) stimulation, recombinant NGF (256-GF, R&D Systems), BDNF (11166-BD, R&D Systems), and NT3 (267-N3-005, R&D Systems) were reconstituted in DMSO or PBS and diluted in media. ..

    Article Title: Longitudinal Characterization of Transcriptomic, Functional, and Morphological Features in Human iPSC-Derived Neurons and Their Application to Investigate Translational Progranulin Disease Biology
    Article Snippet: Neuronal toxicity was induced by treating the cells with staurosporine, a nonselective protein kinase inhibitor (STS, Enzo Life Sciences) at 0.1 μM. .. Vehicle (PBS, Thermo Fisher Scientific, Waltham, MA, USA), 2.2 nM recombinant NGF (Human β-NGF, R&D Systems), 3.7 nM recombinant BDNF (R&D Systems) or a concentration range of PGRN Recombinant Human Progranulin/PGRN (R&D Systems, Thermo Fisher Scientific, Waltham, MA, USA) was co-added to the STS treatment as indicated. ..

    Concentration Assay:

    Article Title: Longitudinal Characterization of Transcriptomic, Functional, and Morphological Features in Human iPSC-Derived Neurons and Their Application to Investigate Translational Progranulin Disease Biology
    Article Snippet: Neuronal toxicity was induced by treating the cells with staurosporine, a nonselective protein kinase inhibitor (STS, Enzo Life Sciences) at 0.1 μM. .. Vehicle (PBS, Thermo Fisher Scientific, Waltham, MA, USA), 2.2 nM recombinant NGF (Human β-NGF, R&D Systems), 3.7 nM recombinant BDNF (R&D Systems) or a concentration range of PGRN Recombinant Human Progranulin/PGRN (R&D Systems, Thermo Fisher Scientific, Waltham, MA, USA) was co-added to the STS treatment as indicated. ..



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    human ngf  (R&D Systems)
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    R&D Systems human ngf
    Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced <t>by</t> <t>stimulation</t> with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , <t>NGF</t> , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.
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    Image Search Results


    Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced by stimulation with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , NGF , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.

    Journal: Nature Communications

    Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis

    doi: 10.1038/s41467-026-70000-6

    Figure Lengend Snippet: Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced by stimulation with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , NGF , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.

    Article Snippet: The mixture was placed in an incubator at 37 °C with 5% CO 2 for 30 min, followed by stimulation with recombinant human NGF (R&D Systems, 256-GF) at a final concentration 0.1 μg/mL (or PBS as negative control) and the resulting mixtures were further incubated for 20 min. Next, anti-CD123-APC (Miltenyi Biotec, 130-115-265) and anti-CD203c-PE (Miltenyi Biotec, 130-092-343) antibodies were added and continued to incubate for another 15 minutes.

    Techniques: RNA Expression, Control, Expressing, RNA Sequencing